GENE-REGULATION OF INTERLEUKIN-1-BETA, INTERLEUKIN-1 RECEPTOR-TYPE-I,AND PLASMINOGEN-ACTIVATOR INHIBITOR-1 AND INHIBITOR-2 IN HUMAN GRANULOSA-LUTEAL CELLS
Gn. Piquette et al., GENE-REGULATION OF INTERLEUKIN-1-BETA, INTERLEUKIN-1 RECEPTOR-TYPE-I,AND PLASMINOGEN-ACTIVATOR INHIBITOR-1 AND INHIBITOR-2 IN HUMAN GRANULOSA-LUTEAL CELLS, Fertility and sterility, 62(4), 1994, pp. 760-770
Objective: To investigate the regulation of messenger ribonucleic acid
(mRNA) levels of interleukin-l beta (IL-1 beta), interleukin-l (IL-1)
receptor type 1, and plasminogen activator (PA) inhibitor-1 and -2 in
cumulus cells, granulosa-luteal cells, and macrophage-depleted granul
osa-luteal cells obtained from human preovulatory follicles. Design: P
rospective longitudinal study. Setting, Patients: Patients undergoing
assisted reproductive technologies (ART) in the Department of Gynecolo
gy and Obstetrics, Stanford University, Stanford, California. Interven
tions: Cumulus cells and granulosa-luteal cells were collected by ultr
asound-guided transvaginal aspiration at the time of ART. Main Outcome
Measures: Northern blot analysis of mRNA levels of IL-1 beta, IL-1 re
ceptor type 1, PA inhibitor-1 and -2 in cumulus cells, granulosa-lutea
l cells and macrophage-depleted granulosa-luteal cells, and indirect i
mmunocytochemical analysis of the IL-1 system and macrophages in granu
losa-luteal cell preparations were performed. Results: Interleukin-1 b
eta mRNA levels in uncultured cumulus cells were less than those of un
cultured granulosa-luteal cells with no differences in IL-1 receptor t
ype 1 mRNA levels between these two cell types. Granulosa-luteal cell
IL-1 receptor type 1 mRNA levels were expressed constitutively through
out 24 hours of culture with no effect by hCG, whereas IL-1 beta mRNA
levels increased within 6 hours, and then remained elevated for 24 hou
rs with no effect by hCG. Interleukin-1 beta significantly increased g
ranulosa-luteal cell mRNA levels of IL-1 beta (over twofold), IL-1 rec
eptor type 1 (over twofold), PA inhibitor-1 (approximately 1.4-fold),
and PA inhibitor-2 (approximately 1.6-fold). In contrast, IL-1 beta ha
d no effect on IL-1 beta and IL-1 receptor type 1 mRNA levels in macro
phage-depleted granulosa-luteal cells. Granulosa-luteal cells, not mac
rophages, account for the majority of the immunocytochemical staining
for IL-1 beta and IL-1 receptor type 1 in follicular aspirates. Conclu
sions: These studies suggest that the IL-1 system is regulated in huma
n granulosa-luteal cells during the periovulatory period. Furthermore,
the augmentation of PA inhibitor-1 and -2 mRNA levels by IL-1 beta su
ggests a potential role for IL-1 beta in remodeling of the human ovary
during the periovulatory period.