ELECTRON-MICROSCOPIC ANALYSIS OF IN-VITRO REPLICATION PRODUCTS OF ORS-8, A MAMMALIAN ORIGIN ENRICHED SEQUENCE

Authors
PEARSON CE SHIHABELDEEN A PRICE GB ZANNISHADJOPOULOS M
Citation
Ce. Pearson et al., ELECTRON-MICROSCOPIC ANALYSIS OF IN-VITRO REPLICATION PRODUCTS OF ORS-8, A MAMMALIAN ORIGIN ENRICHED SEQUENCE, Somatic cell and molecular genetics, 20(3), 1994, pp. 147-152
Citations number
25
Categorie Soggetti
Cytology & Histology","Genetics & Heredity",Biology
Journal title
Somatic cell and molecular genetics → ACNP
ISSN journal
07407750
Volume
20
Issue
3
Year of publication
1994
Pages
147 - 152
Database
ISI
SICI code
0740-7750(1994)20:3<147:EAOIRP>2.0.ZU;2-S
Abstract
Electron microscopy was used to map the initiation site of ors 8 DNA r eplication in vitro in a system that is capable of initiating and supp orting one round of semiconservative replication of cloned mammalian D NA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the mo nkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.