SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FORTHERAPEUTIC MONITORING OF HALOPERIDOL LEVELS

This laboratory has developed a simple and efficient solid-phase extra ction method that is combined with a high-performance liquid chromatog raphic method for rapid and precise therapeutic monitoring of haloperi dol (Haldol) blood levels. The solid-phase extraction utilizes a mixed bed column. Sensitivity of the chromatographic method is 0.5 ng/ml (1 .3 nM) of drug in serum, and separations can be performed in a 15-min chromatographic run. Advantages of this approach include enhanced spee d, sensitivity, and efficiency. A high level of sensitivity may be ach ieved because of the absence of interferences from other drugs, metabo lites, or serum components.