INDUCTION OF CAMP-DEPENDENT PROTEIN-KINASE (PKA) ACTIVITY IN T-CELLS AFTER STIMULATION OF THE PROSTAGLANDIN-E2 OR THE BETA-ADRENERGIC RECEPTORS - RELATIONSHIP BETWEEN PKA ACTIVITY AND INHIBITION OF ANTI-CD3 MONOCLONAL ANTIBODY-INDUCED T-CELL PROLIFERATION

Recently, we have shown that T cells exposed to concentrations of pros taglandin E2 (PGE(2)) or the beta-adrenergic receptor agonist isoprote renol (ISO) that elicit equimolar levels of cAMP accumulation do not i nhibit anti-CD3 monoclonal antibody-induced T cell proliferation to th e same extent. This report extends these studies by investigating the induction of cAMP-dependent protein kinase (PKA) in T cells stimulated with PGE(2) or ISO. The kinetics of PKA activity induced by PGE(2) or ISO in T cells are similar but PGE(2) induces more PKA activity. When T cells were treated with concentrations of PGE(2) or ISO that elicit ed similar PKA activities, PGE(2) was found to be more immunosuppressi ve than ISO. T cells stimulated with PGE(2) or ISO showed similar leve ls of increased PKA activity in both the cytosolic and the particulate fractions. Quantitation of the activity of PKA I and PKA II isozymes in T cells stimulated with PGE(2) or ISO revealed that both types were activated; however, while PGE(2) induced the utilization of an equal amount of both isozymes in T cells, ISO-treated cells utilized twice a s much PKA I compared to PKA II. Overall, these results suggest that q ualitative differences in the concentration of cAMP and PKA activity a re important elements in modulatory T cell proliferative responses. (C ) 1994 Academic Press, Inc.