PSEUDOCONTINUOUS CULTURE OF MARINE RECOMBINANT CYANOBACTERIA UNDER A LIGHT-DARK CYCLE

Pseudo-continuous culture of recombinant marine cyanobacteria was carr ied out under a light dark cycle. As a model foreign gene, the structu ral gene of chloramphenicol acetyl transferase (CAT) was used. Under a light dark alternate conditions, a steady state cell concentration wa s observed. However, the level of CAT expression did not show a statio nary phase, but oscillated with the light dark cycle. This may be due to the lack of energy for foreign peptide synthesis during dark incuba tion. This result also indicates the possibility of regulation of fore ign gene expression by controlling light illumination.