EFFECT OF GRANULOCYTE-COLONY-STIMULATING FACTOR ON PIG-BLOOD PLATELETS TREATED WITH CISPLATIN IN-VITRO

The effects of granulocyte colony stimulating factor (G-CSF) and cispl atin in vitro on the peroxidation of pig platelet endogenous arachidon ate were investigated. Estimation of the level of malonyldialdehyde (M DA) after stimulation of platelets with thrombin served as an indicato r of this process. MDA concentration was determined by means of a modi fied method with thiobarbituric acid. The effects of G-CSF on thrombin -induced formation of MDA in platelets was dependent on the concentrat ion and the time of platelet preincubation with this cytokine. In the platelets obtained from the whole blood preincubated with the highest concentration of G-CSF (8 mu g/ml), a significant increase of MDA form ation was observed (p<0.01). Cisplatin (20 mu M) had a strong inhibito ry effect on arachidonic acid metabolism in platelets (p<0.001). In th e presence of G-CSF (0.8 mu g/ml) in incubation medium the inhibitory effect of cisplatin on platelet arachidonate pathway was reduced. The results showed that G-CSF had a protective effect against cisplatin ac tion on platelets.