CLONING OF THE MOVEMENT PROTEIN GENES OF 2 STRAINS OF TOBACCO MOSAIC-VIRUS AND THEIR EXPRESSION IN ESCHERICHIA-COLI-CELLS

Authors
IVANOV KI IVANOV PA TIMOFEEVA EK EFIMOV VA DOROKHOV YL
Citation
Ki. Ivanov et al., CLONING OF THE MOVEMENT PROTEIN GENES OF 2 STRAINS OF TOBACCO MOSAIC-VIRUS AND THEIR EXPRESSION IN ESCHERICHIA-COLI-CELLS, Bioorganiceskaa himia, 20(7), 1994, pp. 751-758
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
7
Year of publication
1994
Pages
751 - 758
Database
ISI
SICI code
0132-3423(1994)20:7<751:COTMPG>2.0.ZU;2-R
Abstract
Recombinant constructs expressing fusion (His)6 movement proteins of t wo strains (Cruciferous and Vulgare) of tobacco mosaic virus were gene rated by cloning the PCR-amplified fragments into the pQE-9 vector. Th e movement proteins containing N-terminal (His)6 affinity tags were pu rified on a metal chelate adsorbent.