SYNTHESIS AND CLONING OF GENES ENCODING A NTISENSE-PEPTIDES FOR HUMANCALCITONIN AND MINIPROINSULIN

Because of the potential significance of the 'molecular recognition' t heory for studies in molecular biology and biotechnology, the theory i s worth being examined using methods of chemical-enzymatic gene synthe sis, recombinant DNA construction and microbiological peptide synthesi s. We therefore undertook the synthesis of human Va18-calcitonin, mini proinsulin, and the corresponding antisense peptides as model compound s. In designing an experimental system the idea was to combine sense a nd antisense polypeptides into a single chain and to examine their int ramolecular interaction. In this paper the chemical-enzymatic synthesi s, cloning and expression of the genes for calcitonin, miniproinsulin, the corresponding antisense peptides and their combinations are descr ibed. The recombinant DNAs obtained were able to direct in vivo expres sion of the target polypeptides as hybride proteins with the IgG-bindi ng domain of the staphylococcal A protein in bacterial cells.