HIGHLY PURIFIED D1 D2/CYT B559 PREPARATIONS FROM SPINACH DO NOT CONTAIN THE NONHEME IRON CENTER/

Authors
KURRECK J GARBERS A PARAK F RENGER G
Citation
J. Kurreck et al., HIGHLY PURIFIED D1 D2/CYT B559 PREPARATIONS FROM SPINACH DO NOT CONTAIN THE NONHEME IRON CENTER/, FEBS letters, 403(3), 1997, pp. 283-286
Citations number
34
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
403
Issue
3
Year of publication
1997
Pages
283 - 286
Database
ISI
SICI code
0014-5793(1997)403:3<283:HPDDBP>2.0.ZU;2-Y
Abstract
Fe-57 enriched D1/D2/cyt b559 preparations were isolated from spinach grown hydroponically on a Fe-57 containing medium. In terms of polypep tide and pigment composition these samples are of high purity and func tional integrity of P680. Mossbauer spectra measured in D1/D2/cyt b559 complexes revealed that these preparations are completely deprived of the non-heme iron center. Possible implications of this finding are d iscussed for the electron transfer from Pheo(-.) to exogenous electron accepters. (C) 1997 Federation of European Biochemical Societies.