CHROMOSOMAL LOCALIZATION AND CDNA CLONING OF THE HUMAN DBP AND TEF GENES

We have isolated cDNA and genomic clones and determined the human chro mosome positions of two genes encoding transcription factors expressed in the liver and the pituitary gland: albumin D-site-binding protein (DBP) and thyrotroph embryonic factor (TEF). Both proteins have been i dentified as members of the PAR (proline and acidic amino acid-rich) s ubfamily of bZIP transcription factors in the rat, but human homologue s have not been characterized. Using a fluorescence in situ hybridizat ion technique, the DBP locus was assigned to chromosome 19q13, and TEF to chromosome 22q13. Each assignment was confirmed by means of human chromosome segregation in somatic cell hybrids. Coding sequences of DB P and TEF; extending beyond the bZIP domain to the PAR region, were hi ghly conserved in both human-human and interspecies comparisons. Conse rvation of the exon-intron boundaries of each bZIP domain encoding exo n suggested derivation from a common ancestral gene. DBP and TEF mRNAs were expressed in all tissues and cell lines examined, including brai n, lung, liver, spleen, and kidney. Knowledge of the human chromosome locations of these PAR proteins will facilitate studies to assess thei r involvement in carcinogenesis and other fundamental biological proce sses. (C) 1994 Academic Press, Inc.