The bradykinin B-2 receptor (BDKRB2) has high affinity for the intact
kinins, which mediate a wide spectrum of biological effects, including
pain, inflammation, vasodilation, and smooth muscle contraction and r
elaxation. In the present study, we have cloned and sequenced the gene
encoding human bradykinin B-2 receptor from a human genomic library.
The B-2 receptor gene contains three exons separated by two introns. T
he first and second exons are noncoding, while the third exon contains
the full-length coding region, which encodes a protein of 364 amino a
cids forming 7 transmembrane domains. The human Bp gene shares high se
quence identity with rat and mouse Bp receptor genes and significant s
imilarity with the gene encoding the angiotensin II type I receptor in
the nucleotide sequence and exon-intron arrangement. In the 5' flanki
ng region, a consensus TATA box and several putative transcription fac
tor-binding sites have been identified. Genomic Southern blot analysis
showed that the B-2 receptor is encoded by a single-copy gene that wa
s localized to chromosome 14q32 by in situ hybridization. In a Souther
n blot analysis following reverse transcription and polymerase chain r
eaction, the human B-2 receptor was found to be expressed in most huma
n tissues. (C) 1994 Academic Press, Inc.