AML1, AML2, AND AML3, THE HUMAN MEMBERS OF THE RUNT DOMAIN GENE-FAMILY - CDNA STRUCTURE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION

cDNAs corresponding to three human rant domain containing genes, AML1, AML2, and AML3, were isolated and characterized. In addition to homol ogy in the highly conserved rant domain, extensive sequence similariti es were also observed in other parts of the proteins. All three carrie d an identical, putative ATP binding site -GRSGRGKS-, and their C-term inal halves were particularly rich in proline and serine residues. Whi le AML1 cDNAs were cloned by others, AML2 represents a new member, not previously described, of the rant domain gene family, and AML3 was id entified as the human homologue of mouse PEB-P2 alpha A. The chromosom al location of AML1 to chromosome 21q22 was confirmed, while AML2 and AML3 were mapped to chromosome regions 1p36 and 6p21, respectively. An alysis of AML1 and AML2 expression in hematopoietic cell lines reveale d a distinct pattern of expression. (C) 1994 Academic Press, Inc.