MAPPING THE GENE THAT ENCODES PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C-GAMMA-2 IN THE HUMAN AND THE MOUSE

We have mapped the PLCG2 gene, which encodes the enzyme phosphatidyl i nositol-specific phospholipase C-gamma 2. This is one of the phospholi pases responsible for catalyzing the hydrolysis of phosphatidyl inosit ol in response to a great many mitogenic stimuli. PL C-gamma 2 is an e ssential component of the signal transduction pathway between tyrosine kinases and downstream events such as protein kinase C activation and intracellular calcium release. We assigned PLCG2 to human chromosome 16 by amplification within a somatic cell hybrid mapping panel. To pos ition the locus at a much finer resolution, PLCG2 sequences were ampli fied from a chromosome 16-specific somatic cell hybrid panel, which pl aced the gene on the long arm of the chromosome in band 16q24.1, a reg ion that has few known genes. We have hybridized a mouse Plcg2 open re ading frame probe to mouse DNAs from the European Interspecific Backcr oss. The segregation pattern reveals the mouse Plcg2 locus maps to dis tal chromosome 8. (C) 1994 Academic Press, Inc.