COMPARATIVE-EVALUATION OF INITIALLY STATIC AND MOTILE SEMEN EJACULATES FROM FRIESIAN AND MURRAH BUFFALO BULLS FOR PHYSICOMORPHOLOGICAL, BIOCHEMICAL, ENZYMATIC AND MINERAL CONSTITUENTS OF SEMINAL PLASMA

The occurrence of static ejaculates averaged 7.82 and 11.21% based on 115 and 107 collections from 3 Friesian and 3 Murrah bulls respectivel y. The revival rate of static ejaculates was significantly lower in Fr iesian than in Murrah. The values in static ejaculates for sperm densi ty, motility, concentration per ml and per ejaculate and live sperm pe r cent were significantly lower and those of pH and dead/abnormal sper m were higher than in the motile ejaculates. The profiles of enzyme GO T, GPT, AKP, ACP and LDH, although apparently lower in static than in motile samples, did not differ significantly in either of the species. Biochemical and mineral constituents were also more in static than in motile ejaculates of both the species. However, only the levels of pr otein, cholesterol, and acid-soluble and ester phosphates differed sig nificantly between static and motile samples in Murrah. In Friesian, t he total protein and copper contents were significantly lower and the chloride and zinc contents were higher in static than in motile ejacul ates. Levels of fructose, inorganic phosphate, sodium, potassium, iron and manganese did not differ significantly between semen types in eit her of the species. Further, the overall means of ejaculate volume, de ad/abnormal sperm, GOT, protein, choloride and sodium were significant ly (P<0.01) higher and those of sperm concentration, motility, AKP, AC P, LDH, fructose, phosphates, zinc, iron and manganese were lower in F riesian than in Murrah. The remaining traits did not vary between them . These findings suggest the poor quality of static ejaculates and the reasons for different behavioural patterns of sperms of 2 species not ed during in-vitro storage.