NUCLEOTIDE-SEQUENCE OF THE COAT PROTEIN-CODING REGION OF THE POTYVIRUS TOBACCO VEIN-BANDING MOSAIC-VIRUS

The sequence of the 3' 1184 nucleotides of tobacco vein-banding mosaic virus (TVBMV) genome has been determined. It contains a single open r eading frame which encompasses the whole of the coat protein of TVBMV. The sequence of the first 20 amino acids at the N-terminal region of the coat protein has also been determined chemically to be GDDQTVDAGKN VQSNQKQRN. The sequence matches the translation product of the open re ading frame starting with amino acid-271; a glycine residue. Thus the coat protein of TVBMV has a calculated M(r) of 30 210. The 3' non-codi ng region of TVBMV is 185 nucleotides in length. Sequence alignment of the coat proteins or the 3' non-coding regions from TVBMV and other r eported potyviruses indicated that TVBMV is a separate species of the potyvirus genus.