RECIPROCAL MODULATION OF CORTICOTROPIN-RELEASING FACTOR AND INTERLEUKIN-1 RECEPTORS FOLLOWING ETHER-LAPAROTOMY STRESS IN THE MOUSE

Ether-laparotomy stress resulted in a dramatic decrease in specific io dine-125-labeled ovine CRF binding ([I-125]oCRF) in the pituitary at 6 h after the onset of the stress although it did not affect [I-125]oCR F binding in the pituitary at 2 h after the stress. [I-125]oCRF bindin g was unchanged in the frontal cortex after the stress. In contrast, [ I-125]interleukin-1 (IL-1)alpha binding was significantly increased in the pituitary at 2 h after the stress and tended to be higher than no n-stressed levels at 6 h after the stress but was not statistically si gnificant. Ether-laparotomy stress did not affect [I-125]IL-1 alpha bi nding in hippocampus, spleen and testis at any time after the stress. Plasma adrenocorticotropic hormone (ACTH) and corticosterone were incr eased at 2 h after the stress. These data demonstrate complex interact ions between CRF and IL-1 receptors on HPA axis during stress.