SIMPLE MONITORING-SYSTEM FOR R-MEDIATED SITE-SPECIFIC RECOMBINATION ON CHROMOSOMES IN SACCHAROMYCES-CEREVISIAE

A simple measuring system for the frequency of recombinant clones gene rated by R-protein mediated recombination was constructed. By applying the system, the frequencies of recombinant clones due to site-specifi c recombination between two loci on one or two different nonhomologous chromosome(s) were monitored during cultivation. Subsequently, it was found that the frequencies increase nearly in proportion to the cultu re period and are different among the individual two selected loci. Th us, the system is thought to be a candidate for analyzing physicochemi cal situations, such as folding and localization, of chromosomes in th e nucleus.