FERMENTATIVE PRODUCTION OF CIS-4-HYDROXY-(L)-PROLINE BY HELICOCERUS-ORYZAE AND ACROCYLINDRIUM-ORYZAE

The production of hydroxyproline by microorganisms was studied to disc over the chiral source of the side chain moiety of carbapenem. cis-4-H ydroxy-(L)-proline produced by Helicocerus oryzae and Acrocylindrium o ryzae was purified from culture broths. The (1)NMR spectra and [alpha] (D) of purified samples were in agreement with those of standard sampl es. Our finding provided the first evidence for fermentative productio n of cis-4-hydroxy-(L)-proline. The addition of (L)-proline to the cul ture broth of H. oryzae increased the production of cis-4-hydroxy-(L)- proline.