THE INHIBITORY-ACTION OF ALUMINUM ON MOUSE BONE-MARROW CELL-GROWTH - EVIDENCE FOR AN ERYTHROPOIETIN-MEDIATED AND TRANSFERRIN-MEDIATED MECHANISM

Aluminum (Al) has been associated with anemia in chronic renal failure patients under hemodialysis as well as in Al-overloaded animals. In a n attempt to elucidate further the mechanism of Al toxicity we have in vestigated the effect of this ion on erythropoiesis in vitro. Mouse bo ne marrow cells were stimulated in vitro with erythropoietin (Epo) in the presence of Al3+ ion and erythroid colony-forming units were then determined. Results of this study indicate that Al compounds (chloride and citrate) at concentrations as low as 0.37 mu mol Al/l inhibit ery thropoiesis in vitro through a mechanism dependent upon the availabili ty of transferrin to bind to aluminum. This process cannot be reversed by increasing Epo doses. This inhibition only occurs,in the presence of Epo at early stages during the interaction of the hormone with its target cell.