DECONVOLUTION OF OVERLAPPING SKEWED PEAKS

Asymmetrical chromatographic peaks can be well modeled by the exponent ially modified Gaussian model (EMG). In this study, an analytical expr ession is derived to give the model for the sharpened overlapping peak s when the deconvolution is performed by another EMG peak as a sharpen ing function. By means of this model, it can easily be explained what kind of peak shapes can be expected after deconvolution. The validity of the model derived was tested by numerical deconvolution methods-the relaxation and the Fourier deconvolution. The model derived here prov es that it is impossible to conserve the peak shape during deconvoluti on. The integration of the peak areas is almost always burdened with e rror. The model gives an explanation of why side lobes and spurious pe aks emerge during the deconvolution procedure.