M. Sterle et N. Pipan, ANALYSIS OF THE CELL-DEATH IN THE MUCOID EPITHELIUM OF MOUSE STOMACH, Folia histochemica et cytobiologica, 32(3), 1994, pp. 155-160
The death and phagocytosis of the stomach mucoid epithelium cells in 2
-8 day old mice were investigated in relation to the state of cell dif
ferentiation and the cell age. The autoradiographic determination of H
-3-thymidine and cytochemical demonstration of neutral mucosubstances
in secretory granules (TA-UA and PA-TCH-SP methods) were used. During
the migration from the proliferation centers to the surface of the muc
oid epithelium the cell death occurs in two different ways: by apoptos
is and by necrosis. Necrosis is limited to superficial, fully differen
tiated mucoid cells, which - after the cell death has occurred - are d
esquamated into the lumen as fragments of irregular shape. The apoptos
is is very common and takes place in all the regions: from the prolife
ration nests up to the surface. It does not depend on the step of diff
erentiation or the age of the cells. In the phagocytosis of apoptotic
bodies are included neighbouring mucoid cells. The general occurrence
of the apoptosis indicates the important role of this process in the r
egulation of the cell population during the morphogenesis of the stoma
ch epithelium.