Mj. Olszewska et al., IN-SITU HYBRIDIZATION OF 2 NODULE-SPECIFIC GENE-PRODUCTS FROM YELLOW LUPIN, Folia histochemica et cytobiologica, 32(3), 1994, pp. 205-208
We have applied in situ hybridization technique to examine the express
ion of two tissue-specific plant genes represented by cDNA clones: pLN
-13 and pLN-50 during the symbiosis of Lupinus luteus with Bradyrhizob
ium lupini. Both genes are expressed in root nodules and their transcr
ipts are restricted to the infected zone of the nodule as revealed wit
h S-35- or H-3-labelled antisense RNA probes. The number of grains ove
r bacteroid-containing cells after hybridization with H-3-labelled pro
bes differed during three tested phases of nodule development: at the
14th, 21st and 28th day after the infection with B. lupini. As control
, apical root meristems and segments of root hair zones of uninfected
plants were used for incubation with RNA of either sense or antisense
orientation preceeded by RNAse A digestion. The data obtained confirm
that both genes are organ-specific and reveal their developmentally re
gulated expression pattern during the course of root nodule morphogene
sis.