T. Ogawa et al., CHEMOTAXIS OF HUMAN MONOCYTES BY SYNTHETIC PEPTIDES THAT MIMIC SEGMENTS OF PORPHYROMONAS-GINGIVALIS FIMBRIAL PROTEIN, Oral microbiology and immunology, 9(5), 1994, pp. 257-261
Porphyromonas gingivalis strain 381 fimbriae and their synthetic pepti
de segments were assessed for migration-stimulating activity on human
peripheral blood monocytes by multiwell chemotaxis assay. P. gingivali
s 381 fimbrial protein was found to markedly enhance migration of huma
n monocytes. The observed increase in monocyte migration occurred main
ly directed toward a positive stimulus (chemotaxis). Furthermore, lipo
polysaccharides extracted from P. gingivalis 381 were shown to induce
chemotaxis and chemokinesis. It was also revealed that the migration o
f monocytes was increased by specific synthetic peptide segments, FP38
1(61-80) and FP381(171-185), that correspond to GKTLAEVKALTTELTAENQE a
nd DANYLTGSLTTFNGA, respectively, based on the amino acid sequence of
the fimbrial subunit protein proposed by Dickinson et al., and the mig
ration stimulation was ascribed to chemotaxis. Furthermore, within the
amino acid sequences, the LTXXLTXXN sequence may play an important ro
le in binding the organisms to monocytes and macrophages and in the in
duction of migration-stimulating activity.