EFFECT OF GAMMA-INTERFERON ON THE SYNTHESIS OF THE FUNCTIONAL ALTERNATIVE AND TERMINAL COMPLEMENT PATHWAYS BY HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS IN-VITRO
V. Berge et al., EFFECT OF GAMMA-INTERFERON ON THE SYNTHESIS OF THE FUNCTIONAL ALTERNATIVE AND TERMINAL COMPLEMENT PATHWAYS BY HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS IN-VITRO, APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 102(7), 1994, pp. 554-558
The cytokine gamma-interferon (gIFN) has been reported to modulate syn
thesis by endothelial cells (EC) of alternative pathway complement fac
tors (H, I, and B). However, the net effect of gIFN on the synthesis a
nd expression of the functional alternative and terminal pathways has
not yet been reported. EC cultured under serum-free conditions were tr
eated with different concentrations of gIFN and simultaneously co-incu
bated with agarose beads, which activate the alternative pathway. C3b
and the terminal complement complex (TCC) bound to co-incubated beads
were measured by radioimmunoassay using anti-human C3c and TCC antibod
ies. gIFN in concentrations 500-4000 U/ml increasingly reduced the amo
unt of C3b and Tcc detected on the beads. The down-regulating effect o
f gIFN on EC complement biosynthesis may be physiologically relevant b
y locally controlling complement activation.