DIKETOPIPERAZINE FORMATION AND N-TERMINAL DEGRADATION IN RECOMBINANT HUMAN GROWTH-HORMONE

Authors
BATTERSBY JE HANCOCK WS CANOVADAVIS E OESWEIN J OCONNOR B
Citation
Je. Battersby et al., DIKETOPIPERAZINE FORMATION AND N-TERMINAL DEGRADATION IN RECOMBINANT HUMAN GROWTH-HORMONE, International journal of peptide & protein research, 44(3), 1994, pp. 215-222
Citations number
19
Categorie Soggetti
Biology
Journal title
International journal of peptide & protein research → ACNP
ISSN journal
03678377
Volume
44
Issue
3
Year of publication
1994
Pages
215 - 222
Database
ISI
SICI code
0367-8377(1994)44:3<215:DFANDI>2.0.ZU;2-P
Abstract
A new degradation process has been identified that occurs in recombina nt DNA-derived human growth hormone. Non-enzymatic cyclization of the first two amino acids from the N-terminus and subsequent cleavage resu lts in the formation of a diketopiperazine and a truncated variant of rhGH. The truncated protein was separated using hydrophobic interactio n chromatography and identified as desPhe(1)Pro(2)-rhGH using N-termin al sequence analysis, tryptic mapping, and mass spectrometry. (C) Munk sgaard 1994.