The detailed composition of wastewater has significant impact on the b
iological wastewater treatment processes. The carbon sources present g
overn the oxygen uptake rate, the rate of denitrification and the biol
ogical phosphorus uptake rate. In this paper, the respiration rate det
erminations used for the bi- or tri-substrate determinations are coupl
ed to removal capacity in order to develop rate-capacity diagrams, tha
t gives a fuller picture of the wastewater and its influence on the bi
ological processes. The directly degradable carbon source gives high r
eaction rates, but the concentration and thus the capacity can be very
limited. In such a case the overall obtainable removal rate is of mor
e interest than the peak rate. The rate-capacity diagrams are shown fo
r raw, primary settled and primary precipitated wastewater, characteri
zed by detailed investigations of the respiration rates obtainable. It
shows the effect of pretreatment not only on the total concentrations
in the pretreated wastewater, but also in the rate-capacity curves fo
r biological denitrification. The rate-capacity curves are in practice
coupled to the design and operation of the treatment plant. These two
factors determine whether the carbon sources in the influent wastewat
er are used for denitrification (or biological phosphorus removal), fo
r oxidation with oxygen or for sludge production.