An abundant growth of zoogloeal colonies was observed periodically dur
ing some experiments carried out on a bench-scale plant fed with a com
plex synthetic wastewater. The plant had a configuration with an enhan
ced regeneration-denitrification-nitrification (R-D-N) activated sludg
e process for low alkalinity wastewaters. Several operational changes
were undertaken in order to suppress the excessive growth of zoogloeal
colonies. They included: (1) changes in the wastewater composition, (
2) volume changes in the compartmentalization of the anoxic selector,
(3) increase of D.O. concentration in the regeneration and nitrificati
on tanks. Conclusions from the plant operation observations give rise
to a hypothesis that none of the operational changes provoked a signif
icant restriction in the abundant zoogloeal growth. When a temporary i
mprovement was observed, after acclimatization on the new operational
conditions the microorganisms culture returned to its previous state a
nd continued to grow in a wave-mode. The reason for the appearance of
this type of sludge might be closely related to the composition of the
synthetic wastewater.