As biological nitrogen removal is introduced at municipal treatment pl
ants, existing trickling filters can be used for either nitrification
or denitrification. In the latter case, the trickling filters must be
operated in an anoxic mode. A pilot-scale study of the use of tricklin
g filters for post-denitrification has been carried out at Ormanas Sew
age Works, Sweden. The pilot trickling filter (4.5 m high and 0.4 m in
diameter) was fed clarified effluent from a full-scale activated slud
ge process, and crude syrup (a by-product from sugar refining containi
ng mainly sucrose) was added as energy and carbon source for the denit
rification. Furthermore, sodium nitrate was added at times when the ni
trification in the activated sludge process was poor. The pilot plant
was operated for over one year and was found to denitrify at a rate of
0.7 g nitrate-N/m(2), day. The aim of the present investigation was t
o study the microbiology of the anoxic trickling filter.