THE EFFECT OF ANTIPROGESTIN (RU-486) AND PROSTAGLANDIN BIOSYNTHESIS INHIBITOR (NAPROXEN) ON UTERINE FLUID PROSTAGLANDIN-F2-ALPHA CONCENTRATIONS

In the present study the effect of the antiprogestin RU 486 and the pr ostaglandin biosynthesis inhibitor, naproxen, on uterine fluid concent ration of prostaglandin F-2 alpha (PGF(2 alpha)) was investigated. RU 486, 200 mg, was administered two days after the luteinizing hormone ( LH) surge and naproxen, 500 mg, was given every 12th hour five times s tarting 4 days after the LH surge. Uterine fluid was collected in the proliferative phase at ovulation and in the mid-luteal phase in a cont rol and treatment cycle. The amount of PGF(2 alpha) was measured by ga s chromatography - mass spectrometry. In the control cycle, the highes t concentration of PGF(2 alpha) was found in the mid-luteal phase, and the lowest at the time of ovulation. Both RU 486 and naproxen reduced the PGF(2 alpha) concentration in uterine fluid considerably, or to 2 2-25% of that in the control cycle at the time of implantation. PGF(2 alpha) produced by the endometrium is believed to be of importance for the implantation of the blastocyst. Postovulatory treatment with RU 4 86 effectively prevents implantation, probably mainly by inhibiting th e maturation of the endometrium during the secretory phase of the cycl e. It is suggested that the inhibition of PGF(2 alpha) release through the uterine fluid caused by RU 486 may also be of importance.