AN INVESTIGATION ON THE ANTIGENIC CROSS-REACTIVITY OF CALLOSELASMA-RHODOSTOMA (MALAYAN PIT VIPER) VENOM HEMORRHAGIN, THROMBIN-LIKE ENZYME AND L-AMINO-ACID OXIDASE USING ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Indirect ELISA shows that the antibodies to Calloselasma rhodostoma ve nom hemorrhagin (CR-HMG), thrombin-like enzyme (CR-TLE) and L-amino ac id oxidase (CR-LAAO) exhibited strong to moderate cross-reactions with most crotalid and viperid venoms, but only anti-CR-LAAO cross-reacted with the elapid venoms. However, the indirect ELISA failed to detect some antigenic similarities demonstrable by cross-neutralization study . The double-sandwich ELISA for the three anti-C. rhodostoma venom com ponents exhibited a much lower level of cross-reactions that the indir ect ELISA.