MICROSATELLITE GENOME SCREENING - RAPID NONDENATURING, NONISOTOPIC DINUCLEOTIDE REPEAT ANALYSIS

The study of reverse genetics has made it possible for scientists to i solate and identify the genes responsible for such human diseases as c ystic fibrosis and Duchenne- and Becker-type muscular dystrophy. The e xpensive and time-consuming process of detecting restriction fragment length polymorphisms by Southern blotting was the only method availabl e to localize these genes. With the discovery of polymorphic microsate llite sequences in the human genome, the speed and ease of which a gen ome screening can be performed has increased dramatically This paper r eports on advanced methods for detection of microsatellite-repeated se quences making the task of mapping human genes simpler, safer and more economical.