Ja. Beamand et al., INDUCTION OF MIXED-FUNCTION OXIDASE ACTIVITIES IN CYNOMOLGUS MONKEY HEPATOCYTE CULTURES, Toxicology in vitro, 8(4), 1994, pp. 549-550
Previous work has demonstrated that primary rat hepatocyte cultures ma
y be used to screen compounds for induction of xenobiotic-metabolizing
enzyme activities. In this study we have examined the effect of some
known enzyme inducers in cynomolgus monkey hepatocytes. Cynomolgus mon
key hepatocytes were isolated by a wedge perfusion technique and could
be cultured in RPMI 1640 medium for at least 5 days. Hepatocytes were
cultured for 70 hr with 10 mu g Aroclor 1254/ml, 10 mu M beta-naphtho
flavone (BNF), 2 mM phenobarbitone (PB) and 100 mu M Wy-14,643. All fo
ur compounds induced 7-benzoxyresorufin O-debenzylase activity to 160-
280% of control levels, whereas 7-ethoxyresorufin O-deethylase activit
y was induced by WY, PB, BNF and ARO to 165, 215, 605 and 1165% of con
trol, respectively. These results demonstrate that cultured Cynomolgus
monkey hepatocytes respond to some known enzyme inducers and hence ma
y be useful for screening chemicals for induction of xenobiotic metabo
lism in primate liver.