EPIDERMAL CYTOKINES AND SKIN SENSITIZATION HAZARD

The induction phase of skin sensitization is associated with the passa ge of antigen-bearing Langerhans cells (LC) from the epidermis to the draining lymph nodes. Recent investigations have revealed that the ind uction of LC migration following topical sensitization is dependent on tumour necrosis factor alpha (TNF-alpha), an epidermal cytokine. Whil e in transit to the lymph nodes LC are subject to both phenotypic and functional maturation which, by analogy with in vitro studies, is also effected by epidermal cytokines (granulocyte/macrophage colony-stimul ating factor, GM-CSF and interleukin-1, IL-1). It is now apparent that Langerhans cell function, the induction of cutaneous immune responses and effective sensitization are dependent on the availability of such cytokines and that contact allergens are able to provoke their produc tion by keratinocytes and by Langerhans cells themselves. The developm ent of screening strategies for the evaluation of skin sensitization p otential as a function of epidermal cytokine production is discussed.