IN-VITRO TOXICITY OF CEPHALOSPORIN AND AMINOGLYCOSIDE ANTIBIOTICS TO LLC-PK1 CELLS ASSESSED BY CHANGES IN GLUCOSE-UPTAKE

Authors
BLACKMORE M RICHARDSON JC COSTELLO S HARPUR ES GRAY TJB
Citation
M. Blackmore et al., IN-VITRO TOXICITY OF CEPHALOSPORIN AND AMINOGLYCOSIDE ANTIBIOTICS TO LLC-PK1 CELLS ASSESSED BY CHANGES IN GLUCOSE-UPTAKE, Toxicology in vitro, 8(4), 1994, pp. 771-773
Citations number
3
Categorie Soggetti
Toxicology
Journal title
Toxicology in vitro → ACNP
ISSN journal
08872333
Volume
8
Issue
4
Year of publication
1994
Pages
771 - 773
Database
ISI
SICI code
0887-2333(1994)8:4<771:ITOCAA>2.0.ZU;2-C
Abstract
Nephrotoxin-induced damage to cultured kidney cells (LLC-PK1) was inve stigated using assays of general cytotoxicity (neutral red uptake) and of functional impairment (glucose accumulation). The latter correctly ranked both the cephalosporin and aminoglycoside antibiotics (acute a nd chronic proximal tubule toxicants respectively) in terms of their r elative in vivo toxicities. Functional impairment after low-dose long- term exposure to the aminoglycosides occurred at sublethal drug concen trations. Assessment of general cytotoxicity correctly ranked only the aminoglycoside antibiotics.