A MODEL SYSTEM FOR THE CONTINUOUS PRODUCTION OF A HETEROLOGOUS PROTEIN USING A NOVEL SECRETION PROMOTING FACTOR WHICH OPERATES IN ESCHERICHIA-COLI

Authors
TOKUGAWA K ISHII T NAKAMURA K MASAKI H UOZUMI T
Citation
K. Tokugawa et al., A MODEL SYSTEM FOR THE CONTINUOUS PRODUCTION OF A HETEROLOGOUS PROTEIN USING A NOVEL SECRETION PROMOTING FACTOR WHICH OPERATES IN ESCHERICHIA-COLI, Journal of biotechnology, 37(1), 1994, pp. 33-37
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
37
Issue
1
Year of publication
1994
Pages
33 - 37
Database
ISI
SICI code
0168-1656(1994)37:1<33:AMSFTC>2.0.ZU;2-V
Abstract
The 'PAS factor', whose gene has been cloned from a species of Vibrio, is a novel protein secretion factor which is functional in Escherichi a coli cells. To demonstrate that practical use of the PAS factor gene is' possible, we have constructed a model secretion vector 'pAS23'. U sing this system, beta-lactamase was produced and secreted into the me dium of a continuous culture system, after optimization of culture con ditions.