CHRONOBIOLOGICAL ASPECTS OF LYSOSOMAL-ENZ YMES ACTIVITY IN SYNOVIAL-FLUID AND REMISSION OF RHEUMATOID INFLAMMATION

A study of activity of 4 lysosomal enzymes (LE) (deoxyribonucleaze, ac id phosphataze, acid cathepcines and betagalactosidaze) in synovial fl uid (SF) depending on month of obtaining of the sample was conducted i n 112 patientes with rheumatoid arthritis (RA). A statistically signif icant chronobiological difference in this parameter was shown for all investigated enzymes with its increasing in spring and autumn and decr easing in summer and winter. The mean duration of the disease was 9.05 +/- 0.51 years. The dependence of duration of the effect after single intraarticular injection of glucocorticosteroids (methylprednisolone acetate, triamcinolone acetonide, the composition of the latest with- cyclophosphamide and dymethylsulfoxide) upon the month of the puncture was investigated in 194 patients with RA. A statistically significant fluctuation (r<0.05) of remission duration after single injection was shown only for triamcinolone acetonide +/- cyclophosphamide, but the analysis of graphic distribution gives an opportunity to imagine such a chronobiological law for other lysosomal enzymes. The identical dist ribution of extremes of LE activity and duration of effect after singl e intraarticular injection of glucocorticosteroids suggests the chrono biological link of these two parameteres.