EFFECT OF UNMODIFIED TRIPLE HELIX-FORMING OLIGODEOXYRIBONUCLEOTIDE TARGETED TO HUMAN MULTIDRUG-RESISTANCE GENE MDR1 IN MDR CANCER-CELLS

Authors
SCAGGIANTE B MORASSUTTI C TOLAZZI G MICHELUTTI A BACCARANI M QUADRIFOGLIO F
Citation
B. Scaggiante et al., EFFECT OF UNMODIFIED TRIPLE HELIX-FORMING OLIGODEOXYRIBONUCLEOTIDE TARGETED TO HUMAN MULTIDRUG-RESISTANCE GENE MDR1 IN MDR CANCER-CELLS, FEBS letters, 352(3), 1994, pp. 380-384
Citations number
42
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
352
Issue
3
Year of publication
1994
Pages
380 - 384
Database
ISI
SICI code
0014-5793(1994)352:3<380:EOUTHO>2.0.ZU;2-1
Abstract
The human mdr1 gene encodes a transmembrane glycoprotein the over-expr ession of which is associated with development of multidrug resistance in human tumor cells. A negative modulation of human mdr1 has been at tempted via a 27-mer unmodified triple helix-forming oligonucleotide, named 1D, targeted to a homopurine sequence in the coding region of th e gene. By administering 10 mu M of 1D we could find a significant red uction in MDR1 mRNA levels in the human drug-resistant cell line CEM-V LB100. This effect appears to be specific and due to a transient block of RNA polymerase mediated by triple helix formation.