MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF ENZYMATICALLY SYNTHESIZED RNA UP TO 150 KDA

Authors
KIRPEKAR F NORDHOFF E KRISTIANSEN K ROEPSTORFF P LEZIUS A HAHNER S KARAS M HILLENKAMP F
Citation
F. Kirpekar et al., MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF ENZYMATICALLY SYNTHESIZED RNA UP TO 150 KDA, Nucleic acids research, 22(19), 1994, pp. 3866-3870
Citations number
21
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
22
Issue
19
Year of publication
1994
Pages
3866 - 3870
Database
ISI
SICI code
0305-1048(1994)22:19<3866:MLIMOE>2.0.ZU;2-P
Abstract
Enzymatically synthesized RNA samples (in vitro transcripts) were anal ysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MA LDI-MS on complex mixtures. Based on these data, the analysis by MALDI -MS of DNA sequencing reactions, produced by the action of an RNA poly merase, is discussed.