NEW RESTRICTION-ENDONUCLEASE CVIRI CLEAVES DNA AT TG CA SEQUENCES/

Authors
JIN AW ZHANG YP XIA YN TRAYLOR E NELSON M VANETTEN JL
Citation
Aw. Jin et al., NEW RESTRICTION-ENDONUCLEASE CVIRI CLEAVES DNA AT TG CA SEQUENCES/, Nucleic acids research, 22(19), 1994, pp. 3928-3929
Citations number
11
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
22
Issue
19
Year of publication
1994
Pages
3928 - 3929
Database
ISI
SICI code
0305-1048(1994)22:19<3928:NRCCDA>2.0.ZU;2-0
Abstract
A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonu clease to recognize the sequence 5'-TGCA-3' and cleaves DNA between th e G and C residues to produce blunt-end termini. Methylation of the ad enine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detec ting mutant alleles of many heritable human genetic diseases.