REGULATION OF CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC) MESSENGER-RNA PRODUCTION IN CULTURED RAT-CELLS

Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD poly peptide originally purified from media conditioned by interleukin-1 be ta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the n ormal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK ce lls, 49F, we found that lipopolysaccharide (LPS), IL-1 beta, and tumor necrosis factor-alpha (TNF-alpha) each induce synthesis of CINC mRNA and CINC, although in qualitatively and quantitatively different patte rns. Through deadenylation experiments and by probing with oligonucleo tides, we discovered that the smaller of the two major CINC transcript s appears to arise from the larger as a result of poly(A) tail removal and/or 3' cleavage.