SYNAPTIC CONNECTIONS BETWEEN PARS COMPACTA AND PARS RETICULATA NEURONS - ELECTROPHYSIOLOGICAL EVIDENCE FOR FUNCTIONAL MODULES WITHIN THE SUBSTANTIA-NIGRA
M. Hajos et Sa. Greenfield, SYNAPTIC CONNECTIONS BETWEEN PARS COMPACTA AND PARS RETICULATA NEURONS - ELECTROPHYSIOLOGICAL EVIDENCE FOR FUNCTIONAL MODULES WITHIN THE SUBSTANTIA-NIGRA, Brain research, 660(2), 1994, pp. 216-224
Intracellular recordings were performed in vitro from both pars compac
ta and pars reticulata neurones of the substantia nigra, and their pos
tsynaptic responses to electrical stimulation within the nucleus were
analysed. Intracellular staining by biocytin was used to reveal the mo
rphology and location of the recorded neurone and its position and dis
tance to the stimulating electrode. Inhibitory postsynaptic potentials
in pars compacta neurones were evoked exclusively from a specific reg
ion of stimulation within the pars reticulata; this field could be map
ped out as a cone-shaped region surrounding the apical dendrite of the
cell and perpendicular to the plane of the pars compacta. Furthermore
, hemitransection, prior to the experiments which eliminated the most
likely source of extrinsic inhibition (the GABAergic striatonigral pat
hway) affected neither the generation of inhibitory postsynaptic poten
tials nor its topographic pattern during pars reticulata stimulation.
In contrast to the response of dopaminergic pars compacta neurones, pa
rs reticulata neurones responded to stimulation over wide areas of sub
stantia nigra, without any clear site-specific selectivity. It is conc
luded that within the substantia nigra, dopaminergic neurones are arra
nged in functional modules such that most inhibition is derived from a
highly local circuit with the collaterals of adjacent pars reticulata
cells. This module is intrinsic to the substantia nigra and may repre
sent the basic functional unit of the nucleus.