RAPID ANALYSIS OF FREE MEDIUM-CHAIN FATTY-ACIDS AND RELATED ETHYL-ESTERS IN BEER USING SPE AND HRGC

Authors
BATTISTUTTA F BUIATTI S ZENAROLA C ZIRONI R
Citation
F. Battistutta et al., RAPID ANALYSIS OF FREE MEDIUM-CHAIN FATTY-ACIDS AND RELATED ETHYL-ESTERS IN BEER USING SPE AND HRGC, HRC. Journal of high resolution chromatography, 17(9), 1994, pp. 662-664
Citations number
12
Categorie Soggetti
Chemistry Analytical
Journal title
HRC. Journal of high resolution chromatography → ACNP
ISSN journal
09356304
Volume
17
Issue
9
Year of publication
1994
Pages
662 - 664
Database
ISI
SICI code
0935-6304(1994)17:9<662:RAOFMF>2.0.ZU;2-4
Abstract
A modification of a procedure by Hage [1] is proposed for the gas chro matographic evaluation of the content of free medium-chain fatty acids and related ethyl eaters in beer. The method involves extraction of f ree fatty acids and ethyl esters by SPE using C-18 bonded phase column s, derivatization of free fatty acids and related ethyl eaters with di azomethane, and GC analysis using an SP-2340 capillary column. The res ults obtained have shown the method to be rapid and highly reproducibl e. The technique has been compared with other methods used for determi nation of free fatty acids.