CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF ESCHERICHIA-COLI LYSYL-TRANSFER-RNA SYNTHETASE (LYSU)

Authors
ONESTI S THEOCLITOU ME WITTUNG L MILLER AD PLATEAU P BLANQUET S BRICK P PERNILLA E
Citation
S. Onesti et al., CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF ESCHERICHIA-COLI LYSYL-TRANSFER-RNA SYNTHETASE (LYSU), Journal of Molecular Biology, 243(1), 1994, pp. 123-125
Citations number
19
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
243
Issue
1
Year of publication
1994
Pages
123 - 125
Database
ISI
SICI code
0022-2836(1994)243:1<123:CAPDSO>2.0.ZU;2-V
Abstract
Crystals of Escherichia coli lysyl tRNA synthetase (lysU gene product) have been obtained by vapour diffusion techniques. Three different cr ystal forms could be grown under similar conditions. The crystals that have been chosen for the structure determination belong to space grou p C222(1) with cell dimensions a = 144.3 Angstrom, b = 257.8 Angstrom, c = 182.1 Angstrom and contain three monomers in the asymmetric unit. They diffract to at least 2.1 Angstrom resolution, but are very sensi tive to radiation damage.