PROPAFENONE BINDING INTERACTION WITH HUMAN ALPHA(1)-ACID GLYCOPROTEIN- ASSESSING EXPERIMENTAL-DESIGN AND DATA EVALUATION

Binding data on racemic RS-propafenone as well as individual R- and S- drug enantiomers interacting reversibly with human alpha(1)-acid glyco protein, as obtained by a high-performance liquid chromatographic meth od, are evaluated according to three different approaches introduced, respectively, by Scatchard, Bjerrum, and by Tobler and Engel. A non-li near curve-fitting procedure was applied to compute the binding parame ters exclusively for the binary system comprising the examined protein and R- and S-propafenone, individually. The exactness of the study de sign rather than the numerical values were the focus of attention in t he evaluation of the data found.