J. Amedee et al., EVALUATION OF CELL COLONIZATION ON BIOMATERIALS - PREVENTING CELL ATTACHMENT TO PLASTIC CONTAINERS, Biomaterials, 15(12), 1994, pp. 1029-1031
In biocompatibility evaluation involving cell culture models, we use s
amples of biomaterials of different forms and sizes. During cell seedi
ng onto biomaterials of an inadequate size to cover the bottom of the
culture wells completely, cells have the opportunity to attach to the
plastic. As described in this report with two culture models and two b
iomaterials, we use an agarose gel sublayer to prevent this phenomenon
.