MOLECULAR METHODS FOR THE DIFFERENTIATION OF THE EQUINE HERPES-VIRUS VACCINE STRAIN RAC-H

Authors
MEYER H HUBERT P EICHHORN W
Citation
H. Meyer et al., MOLECULAR METHODS FOR THE DIFFERENTIATION OF THE EQUINE HERPES-VIRUS VACCINE STRAIN RAC-H, Tierarztliche Umschau, 49(10), 1994, pp. 624-628
Citations number
17
Categorie Soggetti
Veterinary Sciences
Journal title
Tierarztliche Umschau → ACNP
ISSN journal
00493864
Volume
49
Issue
10
Year of publication
1994
Pages
624 - 628
Database
ISI
SICI code
0049-3864(1994)49:10<624:MMFTDO>2.0.ZU;2-1
Abstract
A method for the rapid differentiation of the EHV1 live vaccine strain Rac H and EHV1 isolates from abortions is described. Total DNA was is olated from virus-infected cell cultures and digested with restriction endonuclease BamHI. A Southern blot was prepared and hybridised with an EHV1-specific probe. Deletions of 0.9 kbp in each of the two fragme nts detected provided the observation of a unique pattern for strain R ac H. Comparison of the different sizes of the hybridised DNA-fragment s revealed that in 75 isolates the live vaccine strain Rac H was not i nvolved.