INFLUENCE OF CULTURE CONDITIONS ON GROWTH AND PROTECTIVE ANTIGENICITYOF CLOSTRIDIUM-CHAUVOEI

The effect of culture conditions on growth and immunogenicity of Clost ridium chauvoei were examined. The pH control and partial feeding of t he carbon source at high concentrations were beneficial for growth. Th e biomass yield was significatively improved, however the butanol conc entration reached toxic levels hampering further growth. For each expe rimental condition the immunogenicity of cells was tested. No differen ces were found with cells obtained at different temperatures, but it d ecreased significatively with the partial supply of the carbon source and pH control.