PROTECTIVE EFFECTS OF LAZAROIDS AGAINST OXYGEN-FREE RADICALS INDUCED LYSOSOMAL DAMAGE

Lipid peroxidation of membranes by oxygen free radicals has been impli cated in various disease states. Different antioxidants and iron chela tors have been used to reduce lipid peroxidation. Lazaroids have been used for the acute treatment of central nervous system disorders such as trauma and ischemia wherein lipid peroxidative processes take place . In this study we evaluated the effect of lazaroids (U-78518F and U-7 4389F) on the release of acid phosphatase activity and formation of ma londialdehyde (MDA) in rat liver lyosomes subjected to exogenously gen erated oxygen free radicals. There was a significant increase in the a cid phosphatase release and MDA formation in the presence of oxygen fr ee radicals. This was prevented by both the lazaroids. In a separate s tudy the effect of lazaroid U-74389F was seen on the zymosan-stimulate d polymorphonuclear (PMN) leukocyte-derived chemiluminescence. The PMN leukocyte chemiluminescent activity was attenuated by the lazaroid in a dose-dependent manner. These studies suggest that lazaroids may inh ibit lipid peroxidation and stabilize the membrane.