EVALUATION OF BACILLUS-SUBTILIS STRAIN IS53 FOR THE PRODUCTION OF BACILLUS-ANTHRACIS PROTECTIVE ANTIGEN

Authors
BAILLIE LWJ JOHNSON M MANCHEE RJ
Citation
Lwj. Baillie et al., EVALUATION OF BACILLUS-SUBTILIS STRAIN IS53 FOR THE PRODUCTION OF BACILLUS-ANTHRACIS PROTECTIVE ANTIGEN, Letters in applied microbiology, 19(4), 1994, pp. 225-227
Citations number
10
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
19
Issue
4
Year of publication
1994
Pages
225 - 227
Database
ISI
SICI code
0266-8254(1994)19:4<225:EOBSIF>2.0.ZU;2-1
Abstract
An asporogenous strain of Bacillus subtilis, IS53, transformed with pl asmid pPA102, produces the protective antigen (PA) component of the tr ipartite toxin of B. anthracis. Addition of yeast extract was required to support growth and PA production in all the media examined. Protec tive antigen expression was down-regulated during exponential growth a nd extracellular proteases caused marked degradation of the mature pro tein.