EARLY MORPHOLOGICAL-CHANGES OF PRIMARY AFFERENT NEURONS AND THEIR PROCESSES IN NEWBORN MICE AFTER TREATMENT WITH CAPSAICIN

Degenerating figures of dorsal root ganglion (DRG) neurons and their c entral and peripheral processes (dorsal root and saphenous nerve) and terminals (central terminals in the superficial dorsal horn and cutane ous nerve of the hind paw dorsal skin) of neonatal mice were examined 30 min, 1, 2 and 5 h, and 2, 3, 5, and 10 days after subcutaneous inje ction of capsaicin on post-natal day 2. Many small DRG neurons showed degeneration Ih after treatment. Scarcely any features of degeneration were seen in the DRG and dorsal root 10 days after treatment. The deg enerating aspects of terminal axons in the marginal layer of the super ficial dorsal horn were characterized by enlarged round axons with clo sely packed osmiophilic materials, lamellar bodies, and loss of axopla smic organelles. Two types of central terminals (C-terminals) showed d egeneration in the substantia gelatinosa from 30 min after treatment o nward. One type consisted of small, round, sinuous or slender dark ter minals (CI-terminals), and the other of large, pale, round or angular terminals (CII-terminals). Those that degenerated markedly had homogen eously electron-dense axoplasm with dilated synaptic vesicles and incl usion bodies. Extensive degeneration of terminal axons in the marginal layer occurred 5 h after treatment, whereas conspicuous degeneration of C-terminals occurred from 30 min to 10 days after treatment in the substantia gelatinosa. CI-terminals showed marked degeneration during the first 3 days, whereas marked degeneration of CII-terminals occurre d between 5 and 10 days after treatment. This time difference between the peaks of degeneration of CI- and CII-terminals indicates an import ant difference in the origins of these two types of capsaicin-sensitiv e, nociceptive fibers in the superficial dorsal horn; CI-terminals are derived from small DRG cells, whereas CII-terminals are derived from larger DRG cells. Unmyelinated axons in the dorsal root, saphenous ner ve, and dorsal skin of the hind paw showed similar degeneration patter ns 2 h after treatment to those of terminal axons in the marginal laye r. Thus, the degenerating profiles in the marginal layer suggest that these axons arose from collaterals of unmyelinated primary axons desce nding or ascending within the marginal layer. Numerous enlarged degene rating axons showing vacuolation were conspicuous in the dorsal skin 3 days after treatment. The synchronous degeneration of the smaller DRG neurons, their central and peripheral processes, and their CI-termina ls in the substantia gelatinosa supports the idea that the smaller DRG neurons are directly influenced by capsaicin, and that their degenera tion is followed by centrifugal degeneration.